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- W2390773884 abstract "To investigate the immunoprotection of 66-68 kDa soluble antigen of immature egg of Schistosoma japonicum(SIEA66-68kDa) and to evaluate its feasibility of being used as a molecular vaccine antigen,the SIEA66-68kDa was prepared through SDS-PAGE electrophoresis,protein band resection,electro-elution and ultrafiltration,and the isolated product of protein was used as antigen to immunize Kunming strain of mice.After 3 courses of immunization,mice were challenged with cercariae of S.japonicum,and the reduction rates of worms and eggs were compared with those of the control group with inoculation of PBS.It was found that the mono-specific antibody response against SIEA66-68kDa protein could be induced in mice immunized with this protein.The SIEA66-68kDa could induce significant reduction in adult worms(41.70%).The rates of egg reduction in the SIEA66-68kDa group reach 51.23%,59.26% and 45.17% in the liver,intestine tissue and feces,respectively,with significant difference when compared with the control group.It is concluded that the 66-68 kDa immature egg antigen of S.japonicum could protect the Kunming strain of mice from the infection of this parasite,and it could be regarded as the target antigen for the preparation of vaccine against S.japonicum infection." @default.
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- W2390773884 date "2011-01-01" @default.
- W2390773884 modified "2023-09-22" @default.
- W2390773884 title "Isolation and purification of the 66-68 kDa soluble immature egg antigen Schistosoma japonicum and its protective immunity" @default.
- W2390773884 hasPublicationYear "2011" @default.
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