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- W2390787441 abstract "Objective:Based on atenolol,metoprolol and esmolol can remarkably enhance tris(2,2′-bipyridyl) ruthenium(Ⅱ)(Ru(bpy)32+) electrochemiluminescence(ECL) intensity,a novel approach for the separation and determination of metoprolol tartrate was established using capillary electrophoresis coupled with end-column ECL detection.Methods:Parameters affecting separation and detection were optimized by adding poly-β-cyclodextrin(poly-β-CD) into running buffer,respectively,the optimized separation conditions were 20 mmol·L-1 pH 10.0 phosphate solution containing 10 mg·mL-1 poly-β-CD as running buffer,a separation potential of 9 kV,a sample injection of 5.0 s at 7 kV.In detection pool,50 mmol·L-1 pH 8.5 phosphate was used as running buffer,the potential of work electrode was 1.17 V.Results:Under the optimized experiment conditions,atenolol,metoprolol and esmolol could be baselinely separated and detected within 14 minutes in this method.The ECL intensity were linear with three medicines concentration in the range of 2.5×10-6-1.25×10-4,5.0×10-7-2.5×10-5 and 2.5×10-6-1.25×10-4 mol·L-1,respectively.And the detection limits(S/N=3) of three medicines were 5×10-7,1×10-7 and 5×10-7 mol·L-1,respectively.Conclusion:The developed method is high sensitivity and convenient,which has been successfully applied to detect metoprolol tartrate in betaloc tablets." @default.
- W2390787441 created "2016-06-24" @default.
- W2390787441 creator A5030043713 @default.
- W2390787441 date "2011-01-01" @default.
- W2390787441 modified "2023-09-24" @default.
- W2390787441 title "Separation and determination of metoprolol tartrate with capillary electrophoresis and electrochemiluminescenece detection" @default.
- W2390787441 hasPublicationYear "2011" @default.
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