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- W2390877314 abstract "Objective An HPLC method was developed for determination of docetaxel in rat plasma.Methods The rat plasma samples were prepared by applying a liquid-liquid extraction.Diazepam was selected as internal standard.The compounds were eluted on a Diamonsil reverse phase column.The mobile phase consisted of a mixture of water,methanol,tetrahydrofuran,and ammonium hydroxide in a gradient mode at a flow rate of 1.0 mL·min-1.The pH of the solution was adjusted with formic acid.Ultraviolet detection wavelength was set at 232 nm.Results Good linearity was observed over the concentration ranges of 50-10 000 μg·L-1 in rat plasma.The limit of quantification for docetaxel was 50 μg·L-1.The extraction recoveries of docetaxel were 70.2%79.0% from rat plasma.Conclusions The described assay method is quick,simple,and accurate.It has been successfully applied to the pre-clinical pharmacokinetic study of docetaxel." @default.
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- W2390877314 date "2008-01-01" @default.
- W2390877314 modified "2023-09-23" @default.
- W2390877314 title "Determination of docetaxel in rat plasma by RP-HPLC" @default.
- W2390877314 hasPublicationYear "2008" @default.
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