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- W2390881444 abstract "White spot syndrome virus(WSSV))is the largest known animal virus,which has a 300kb circular double-strand DNA genome encoding 532open reading frames(ORF).VP95is a structural protein of WSSV which encode by ORF WSV442of 2 400 nucleotides.In present study,a time course analysis showed that this transcript was actively transcribed at the late stage.To investigate this structural protein,apart of vp95 gene(1 051-1 650nt)was cloned from WSSV genome and then was cloned into pET-His prokaryotic expression vector.The recombinant vector was transferred into Escherichia coli strain BL-21(DE3)and the transferred E.coli were induced by IPTG in order to acquire recombinant fusion protein which was subsequently purified by Ni 2+-NTA agaros. The purified protein was used as antigen to immune the rats.To remove the impurities in the serum,we have chosen the prepackaged column HiTrap NHS-activated HP(GE Healthcare)for preparative affinity chromatography.The immunological specificity of both the unpurified serum and the purified antibody were validated by western blotting.This study has provided an important foundation for further study of the role of VP95in the package of virion and its function in the viral invasion." @default.
- W2390881444 created "2016-06-24" @default.
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- W2390881444 date "2014-01-01" @default.
- W2390881444 modified "2023-09-23" @default.
- W2390881444 title "Transcriptional Analysis of the vp 95 Gene of White Spot Syndrome Virus and Preparation of Polyclonal Antibody of the Structural Protein" @default.
- W2390881444 hasPublicationYear "2014" @default.
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