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- W2390882972 abstract "One-day-old broiler breeders were inoculated with strain SNV of REV and slaughtered at different period.The process of lesion and tumor were study by pathohistologic detection.The distribution of antigen and virus in different organs were detected by immunohistochemistry and PCR.Tumor cell and morphous of virus were observed by electron microscope.The results showed that tumor nodule emerged earlier in liver,spleen,thymus and bursa than in other organs.And the most positive signal were found in these organs.At 5 week,positive signal emerged in cardiac muscle,proventriculus,lung,kidney and bone marrow.But the positive signal was relatively weaker.After 9 weeks,the positive signal declined.LTR of REV was detected by PCR in liver,spleen,thymus,bursa,proventriculus and bone marrow.Result of microscope observation showed that virus distributed in cytoplasm and cell spaces.It was discovered that positive reaction of IHC had relationship with tissue which had typical lesion.Tumor nudules were easily formed in the organ of stronger positive antigennicity,indicating that these organs have the target cell of REV.Though the positive signal was found later in proventricuus,90 percent of proventriculus were intumesce.This implied that proventriculus might be the major target organ of REV." @default.
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- W2390882972 date "2008-01-01" @default.
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- W2390882972 title "Dynamic observation on pathohistologic lesions and antigen location by PCR in broiler breeder infected with REV" @default.
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