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- W2390889658 abstract "γH2AX foci has been generally accepted as a biomarker of DNA double-strand break(DSB).To investigate the feature and characteristics of H2AX phosphorylation(γH2AX) related to cell cycle progression,HeLa cells were synchronized at prometaphase by thymidine double-blocking followed by nocodazole treatment.Cell cycle and protein expression level were analyzed by flow cytometry and Western blotting,respectively.γH2AX foci were detected by fluorescent microscopic observation of immunostaining.The expression level of γH2AX was remarkably increased when the cells entered G2/M and mitotic process.A certain portion of mitotic cells was with a great number of γH2AX foci even without any DNA DSB.As cells exited from M phase,γH2AX expression level was decreased.This G2/M phases-related γH2AX expression pattern was similar to oscilation of G2/M regulators PLK1 and cyclin B1 expression.With 4 Gy of large dose γ-ray irradiation,the cells were arrested in G2/M phases at 8 ~ 12 hours after irradiation.At the 8 hours time point,γH2AX foci number also reached the second peak after the first peak formed at 1 hour post-irradiation.However,with 1 Gy of low dose irradiation,G2/M arrest was very weak,and the number of γH2AX foci peaked at 0.5 hour after the irradiation,and then declined with the time.The timing of γH2AX foci formation and disappearance is consistent with the repair kinetics of ionizing radiation-induced DNA DSB.The results indicated that the cell cycle change should be taken into account when γH2AX is considered as DSB biomarker.However,γH2AX foci can be taken as an indicator of DSB when cells are irradiated with a dose of 1 Gy or lower." @default.
- W2390889658 created "2016-06-24" @default.
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- W2390889658 date "2012-01-01" @default.
- W2390889658 modified "2023-09-25" @default.
- W2390889658 title "Phosphorylation of H2AX Related to the Cell Cycle G_2/M Progression" @default.
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