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- W2390919313 abstract "Aim To express HES1 and prepare its specific antibodies for investigation of its expression in tumors. Methods GST HES1 fusion protein and GST were induced to express in E. Coli , then were purified with glutathione Sepharose 4B. Purified GST HES1 was used to immunize rabbit and purified GST was coupled with CNBr activated Sepharose 4B. The rabbit anti HES1 serum was harvested and the anti GST antibodies in the serum were absorbed with GST Sepharose 4B. The specificity of the ployclonal anti HES1 antibody was determined by indirect ELISA and Western blot. The expression of HES1 in gastric cancer, colon cancer and corresponding non tumor tissues were detected by Western blot. Results GST HES1 fusion protein was expressed and purified successfully. Prepared ployclonal anti HES1 antibody could recognize native HES1 and showed high specificity against HES1 without cross reaction with GST or E. Coli component in ELISA and Western blot. Preliminary western blot result indicated that the expression of HES1 in gastric cancer and colon cancer was significantly higher than that in corresponding non tumor tissues. Conclusion HES1 successfully expressed in E.coli and purified. Bloyclonal anti HES1 antibody with high specificity is prepared and applied successfully in preliminary investigation." @default.
- W2390919313 created "2016-06-24" @default.
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- W2390919313 date "2002-01-01" @default.
- W2390919313 modified "2023-09-23" @default.
- W2390919313 title "Prokaryotic expression of HES1 and preparation of its polyclonal antibody" @default.
- W2390919313 hasPublicationYear "2002" @default.
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