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- W2390934584 abstract "Objective To develop the detection method of low-density gene chip to detect exogenous genes in transgenic soybeans simultaneously.Method Three exogenous DNA fragment,including camv35s promoter,nos termination and cp4 epsps gene,and endogenous gene lectin were selected as target genes,the primers and probes of which were designed and synthesized,then the probes were immobilized on the chip.Multiplex PCR was used to amplify the target sequences from the soybean sample meanwhile the Cy5-dUTP was adulterated into amplicons,which were hybridized with DNA microarray to evaluate the sensitivity.Results The results showed that the DNA chip for identifying various RRS Certified Reference Materials was highly specific and repeatable and the detection level of the exogenous genes in RRS was 0.45%.Conclusion The DNA microarray procedure might help to develop a method with high thoroughput and sensitivity in detecting transgenic soybean." @default.
- W2390934584 created "2016-06-24" @default.
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- W2390934584 date "2008-01-01" @default.
- W2390934584 modified "2023-09-23" @default.
- W2390934584 title "Study on Detection Method of Low-density Gene Chip of Transgenic Soybeans" @default.
- W2390934584 hasPublicationYear "2008" @default.
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