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- W2390935691 abstract "Objective:To determinate the nucleotides sequence of Der f 1 and get know of information on its molecular evolution.Methods:According to the complete CDS encoding the major house dust mite allergen Der f 1 in Genbank,we de- signed the primers and amplified the gene by nest PCR.After purification and recycling,the cDNA fragment was cloned into pMD19-T vector and transformed into E.coli JM109.Then the positive clones were screened and sequenced.After sequence blast, phylogenetic tree was comtructed by Clustal W 1.83.Results:The cDNA fragments encoding Der f1 were amplified successfully, and one open reading fragment(ORF)was found in the sequence with full length of 966 bp.The nucleotide identity with the ref- erence sequence in Genbank was 99.9%.By InterProScan,three cysteine peptidase active sites were found in the sequence.And ac- cording to the phylogenetic tree,Dermatophagodes farinae in this study is nearer related to Euroglyphus maynei and farthest from Drosophila pseudoobscura.Conclusion:The gene fragment encoding the major dust mite allergen Der f1 was amplified success- fully,however,the evolution relationship deduced from phylogenetic tree is not consistent with morphological classification." @default.
- W2390935691 created "2016-06-24" @default.
- W2390935691 creator A5002209420 @default.
- W2390935691 date "2007-01-01" @default.
- W2390935691 modified "2023-09-23" @default.
- W2390935691 title "Sequencing and Polygenetic Analysis of the Major House Dust Mite Allergen,Der f 1" @default.
- W2390935691 hasPublicationYear "2007" @default.
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