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- W2390958595 abstract "Protoplasts of orange-heading chinese cabbage were isolated from cotyledons and hypocotyls.The different factors affecting the protoplasts yield and different methods of purification were investigated.The best enzyme solution of cotyledons contained CPW salts,DPD medium,0.8% cellulase R-10,0.1% macerozyme R-10,0.5 mol/L mannitol incubated at 27℃ for 2 hours.The protoplasts yield was 3.52 ×106/(g FW) and the viability was 60.3% under the condition.The best enzyme solution of hypocotyls contained CPW salts,DPD medium,1.5% cellulase R-10,0.3% macerozyme R-10,0.5 mol/L mannitol incubated at 27℃ for 18 hours.The protoplasts yield was 2.20×106/(g FW) and the viability was 63.7% under the condition.Moreover,the best protoplasts purification method was rising method with sucrose isodensity centrifugation at concentration of 30%,with the.centrifugal speed of 800 rpm,and the centrifugal time of 5 min." @default.
- W2390958595 created "2016-06-24" @default.
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- W2390958595 date "2009-01-01" @default.
- W2390958595 modified "2023-09-25" @default.
- W2390958595 title "The Proplast Isolation and Purification of Orange-heading Chinese Cabbage" @default.
- W2390958595 hasPublicationYear "2009" @default.
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