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- W2390989025 abstract "Objective:To investigate the cardiac stem cell amplification in brown adipose tissue(BAT) in rats with cold temperature stimulation. Methods:The experimental rats were divided into two groups according to breeding temperature as 4℃ group and 24℃ group,n=6 in each group.After 4 weeks breeding,rats' interscapular BAT was isolated and BAT specific protein UCP-1 expression was examined.BAT-derived cells were obtained and the related cell phenotypic character was tested by flow cytometry.With 4 weeks in vitro culture,cardiomyocytes specific antigen was detected,and the efficiency of cell differentiation was compared at different inoculation density. Results:After 4 weeks breeding,in 4℃ group,the expression of UCP-1 protein and the expression of BAT-derived cell phenotypes CD133,CD29 and CD29/CD133 were significantly increased than those in 24℃ group,P0.01.After 4 weeks in vitro culture,in 4℃ group,the cell differentiation efficiency reached(27.3±2.8)% at certain inoculation density which was higher than that in 24℃ group.A positive cardiac-specific gene expression was confirmed by RT-PCR. Conclusion:More cardiac stem cells exist in cold temperature stimulated BAT,which suggesting that the temperature may affect homing and differentiating of cardiac stem cell at certain point." @default.
- W2390989025 created "2016-06-24" @default.
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- W2390989025 date "2011-01-01" @default.
- W2390989025 modified "2023-09-24" @default.
- W2390989025 title "Cold Temperature Stimulating Cardiac Stem Cell Amplification in Rats'Brown Adipose Tissue" @default.
- W2390989025 hasPublicationYear "2011" @default.
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