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- W2390991257 abstract "A human ovarian cancer cell line,HO CIITA constantly expressing the CIITA gene was established and selectively cloned after transfection with pLXN/CIIYA retrovirus The expression levels of HLA molecules and antigen processing associated genes were detected with flow cytometry and RT PCR respectively The CD4 +/CD8 + T cell populations were separated from human PBL by means of magnetic beads, mixed lymphocyte reaction (MLR) between CD4 +T cells and the HO CIITA cell line was performed as usual and ELISA and RT PCR were used to analyze the expressions of IL 4 and IFN γ from the supernatants of cell cultures Experimental results showed that the expressions of HLA II molecules on the HO cells as well as that of the LMP 7 gene were elevated after transfection with the CIITA gene,but the expression of the Ii gene turned from positive to negative No expression of TAP 1 could be found On MLR,it was found that both HO and HO CIITA cells could stimulate the secretion of IL 4 with peak after stimulation for 48 hours,but the concentration of IL 4 was 1:2 between HO and HO CIITA cells There was no obvious change in the secretion of IFN γ Results of RT PCR were in accordance with those of ELISA These results suggest that the surface expression of MHC II antigens in tumor cell line could be enhanced by transfection with CIITA gene with a synergistic effect of IFN γ and an inducing effect for CD4 +T cells to secrete IL 4" @default.
- W2390991257 created "2016-06-24" @default.
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- W2390991257 date "2003-01-01" @default.
- W2390991257 modified "2023-09-23" @default.
- W2390991257 title "Effects of Transfection with MHC Class II Transactivator(CIITA) to Human Ovarian Cancer" @default.
- W2390991257 hasPublicationYear "2003" @default.
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