Matches in SemOpenAlex for { <https://semopenalex.org/work/W2391004804> ?p ?o ?g. }
Showing items 1 to 47 of
47
with 100 items per page.
- W2391004804 abstract "【Objective】To determine the probability of 2D-DIGE technology to identify the differentially expressed proteins in sweet oranges induced by Citrus tristeza virus,CTV.【Method】 The same weight leaf samples of each plant were mixed from CTV-inoculated sweet orange plants and healthy plants,respectively.Total proteins were extracted from above mixed samples with three replications.Each protein sample was labeled with three different CyDyes Cy2,Cy3 and Cy5,and Cy2-labeled sample was used as an internal standard pooled from all the samples.Labeled protein samples were separated with 2-D DIGE and differential protein spots were picked out.MALDI-TOF-MS and bioinformatics were adopted to identify and interpret the significance of differentially expressed proteins.【Result】Total 91 Differential protein spots were detected with statistical variance of two groups(relative average volume ratio ≥1.5;t-test,P0.05).Among these proteins,56 protein volumes of CTV group were higher than that of healthy group,and 35 proteins lower.PMFs of 37 proteins were obtained by MAIDI-TOF-MS analysis.Through searching NCBI,19 protein functions were clear,16 proteins predicted or putative,and 2 proteins unknown.The identified proteins were involved mainly in metabolism(including 2-Phospho-D-glycerate hydrolase,NADP-isocitrate dehydrogenase,Xyloglucan endotransglycosylase,Adenylate kinase),antioxidant activity(including Thioredoxin H-type 5,Ferredoxin-NADP reductase,L-ascorbate peroxidase T,Iron superoxide dismutase),photosynthesis(including Ribulose 1,5-bisphosphate carboxylase/oxygenase large or small subunit,Oxygen-evolving enhancer protein 1 and 2,Chlorophyll a/b-binding protein precursor) and molecular chaperone(such as Heat shock protein).【Conclusion】 2D-DIGE can be used to identify the differentially expressed proteins in sweet oranges induced by different pathologic CTV strains in order to clarify the mechanism of CTV pathogenesis." @default.
- W2391004804 created "2016-06-24" @default.
- W2391004804 creator A5007014008 @default.
- W2391004804 date "2013-01-01" @default.
- W2391004804 modified "2023-09-23" @default.
- W2391004804 title "Identification of differentially expressed proteins in sweet oranges induced by Citrus Tristeza Virus using 2D-DIGE" @default.
- W2391004804 hasPublicationYear "2013" @default.
- W2391004804 type Work @default.
- W2391004804 sameAs 2391004804 @default.
- W2391004804 citedByCount "0" @default.
- W2391004804 crossrefType "journal-article" @default.
- W2391004804 hasAuthorship W2391004804A5007014008 @default.
- W2391004804 hasConcept C104292427 @default.
- W2391004804 hasConcept C104317684 @default.
- W2391004804 hasConcept C153911025 @default.
- W2391004804 hasConcept C55493867 @default.
- W2391004804 hasConcept C86803240 @default.
- W2391004804 hasConceptScore W2391004804C104292427 @default.
- W2391004804 hasConceptScore W2391004804C104317684 @default.
- W2391004804 hasConceptScore W2391004804C153911025 @default.
- W2391004804 hasConceptScore W2391004804C55493867 @default.
- W2391004804 hasConceptScore W2391004804C86803240 @default.
- W2391004804 hasOpenAccess W2391004804 @default.
- W2391004804 hasRelatedWork W2036177483 @default.
- W2391004804 hasRelatedWork W2054948779 @default.
- W2391004804 hasRelatedWork W2078717914 @default.
- W2391004804 hasRelatedWork W2079638934 @default.
- W2391004804 hasRelatedWork W2090381864 @default.
- W2391004804 hasRelatedWork W2091213881 @default.
- W2391004804 hasRelatedWork W2106489617 @default.
- W2391004804 hasRelatedWork W2147657020 @default.
- W2391004804 hasRelatedWork W2236539063 @default.
- W2391004804 hasRelatedWork W2269776938 @default.
- W2391004804 hasRelatedWork W2365890614 @default.
- W2391004804 hasRelatedWork W2369711773 @default.
- W2391004804 hasRelatedWork W2372176619 @default.
- W2391004804 hasRelatedWork W2383498857 @default.
- W2391004804 hasRelatedWork W2387446320 @default.
- W2391004804 hasRelatedWork W2392256788 @default.
- W2391004804 hasRelatedWork W2393487975 @default.
- W2391004804 hasRelatedWork W2592778921 @default.
- W2391004804 hasRelatedWork W28027882 @default.
- W2391004804 hasRelatedWork W792415081 @default.
- W2391004804 isParatext "false" @default.
- W2391004804 isRetracted "false" @default.
- W2391004804 magId "2391004804" @default.
- W2391004804 workType "article" @default.