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- W2391011036 abstract "Objective To study the expression of astrocytoma genes and characterization classification genes by using cDNA microarray. Methods BioStarH140S microarray (including 5 592 novel genes) were used and hybridized with probes, which were prepared for total RNA. It was extracted from 2 cases of normal adult brain tissues and 18 cases of glioma tissues. Differentially expre-ssed genes between normal tissues and glioma tissues were assayed after scanning cDNA microarray with ScanArray 4 000. The gene's characterizations were studied with Hierarchical method. Results Among the 13 939 target genes, there were 438 (3.14%) differentially expressed genes between astrocytoma and normal brain tissue. By bioinformal analysis, those genes were associated with cell signal, cytoskeleton, oncogenes and suppressor genes, etc. MAP7, DBCCR1, PCDHA5, KCNAB1, NAP1L2 genes were relevant to classification by Hierarchical method. Molecular classification was in accordance with pathology and revealed internal essence in tumorigenesis. Conclusion cDNA microarray is an available technique in screening marker genes of glioma and understanding its molecular mechanism and is helpful to diagnosis and therapy of the glioma." @default.
- W2391011036 created "2016-06-24" @default.
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- W2391011036 date "2004-01-01" @default.
- W2391011036 modified "2023-09-25" @default.
- W2391011036 title "Gene Expression and Cluster Analysis of Astrocytoma by Using cDNA Microarray" @default.
- W2391011036 hasPublicationYear "2004" @default.
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