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- W2391014778 abstract "A SYBR Green I based Real-time RT-PCR assay was developed for detection of canine distemper virus(CDV) using a pair of primers derived from the nucleocapsid protein(NP) gene sequence.The specificity,sensitivity and reproducibility of the assay were evaluated and compared with conventional RT-PCR and Colloidal gold immunoassay(GIA).The result showed that the Real-time RT-PCR assay was highly specific and had a broad linear detecting range(5.2×102-5.2×107 copies,R=0.999) with 96.8 % PCR amplification efficiency.It had a detection threshold of 52 copies of plasmid DNA and was 103 and 105 folds more sensitive than the conventional RT-PCR and GIA,respectively.The assay was highly reproducible and had an intra-assay and inter assay coefficient of variations(CVs) of 0.51 %-1.90 % and 1.96 %-2.63 %,respectively.Test on positive clinical samples showed 100 % consistency.The real time PCR assay was used to quantify CDV in the multivalent live attenuated vaccines and showed that those vaccines contained 1.24×105-4.88×105 CDV viruses per dose.The assay provided an effective means for rapid early diagnosis,vaccine quality control and molecular epidemiological investigation of CDV." @default.
- W2391014778 created "2016-06-24" @default.
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- W2391014778 date "2008-01-01" @default.
- W2391014778 modified "2023-09-26" @default.
- W2391014778 title "Development of a SYBR GreenI Real-time RT-PCR assay for detection of canine distemper virus" @default.
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