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- W2391053044 abstract "Objective To establishment a defined three-dimensional culture system of cardiomyocytes from mouse embryonic stem cell-derived embryoid bodies(EBs).Methods The EBs were derived from mES by suspending culture and were plated to MatrigelTM Matrix three-dimensional system.The mouse fibroblast cells served as negative control to identify genes expression of contracting EBs.Results The first beating was irregular after 5 days of culture,but became consistent after extra 3 days with at an average frequency of 80～100 beats per minute.mES expressed cardiac specific protein of cTnT,Desmin and Actin.The expression of genes associated cardiomyocytes differentiation as cTnT,NKX2E,GATA-4 and MLC-2v were detected by RT-PCR.Conclusion By using the MatrigelTM three-dimensional system,mES-derived EBs may spontaneously differentiate to cardiomyocytes effectively,and possess necessary biological characters of cardiomyocytes." @default.
- W2391053044 created "2016-06-24" @default.
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- W2391053044 date "2009-01-01" @default.
- W2391053044 modified "2023-09-26" @default.
- W2391053044 title "Establishment of a cardiomyocyte line from mouse embryonic stem cell-derived embryoid bodies" @default.
- W2391053044 hasPublicationYear "2009" @default.
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