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- W2391096170 abstract "The effects of different concentrations of growth factors,hormones and various culture conditions on growth of the primary cultured cells derived from fins,gills and kidney in turbot Scophthalmus maximus were evaluated in vitro based on the attachment rate,cells emigration rate and cell growth.The hydrocortisone(HC),Epidermal Growth Factor(EGF),insulin(Ins)and sp2/0 conditioned medium were showed to have the potent promoting effects on the primary cultured cells derived from the tissues and organs.The maximum attachment rates and emigration rates were found in the fins and gills for the HC at 0.4 μg/mL,for the EGF at 10 ng/mL,for the Ins 5 μg/mL and for the sp2/0 conditioned medium at 20%-25% in the culture medium.The maximum growth of kidney primary cultured cells was observed for the HC at 0.8 μg/mL,for the EGF at 10 ng/mL,for the Ins at 0.8 μg/mL,and for the sp2/0 conditioned medium at 20% in the culture medium." @default.
- W2391096170 created "2016-06-24" @default.
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- W2391096170 date "2009-01-01" @default.
- W2391096170 modified "2023-09-26" @default.
- W2391096170 title "The effects of growth factors and hormones on growth of the cultured cells in turbot Scophthalmus maximus in vitro" @default.
- W2391096170 hasPublicationYear "2009" @default.
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