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• W2391114897 abstract "Industrial aquaculture of Trionys sinensis has developed rapidly due to the high nutrient content and economic value of the species,but it has also led to a higher incidence of disease. The abuse of antibiotics on Trionys sinensis creates problems,such as bacterial resistance and drug residues,that negatively affect the healthy development of this aquaculture sector. Probiotics,as a new dietary supplement that are non-toxic,don't lead to resistance and leave no residue could,to some extent,replace antibiotics. The aim of this investigation was to evaluate the effects of compound probiotic dose on the growth rate and blood biochemistry of Trionys sinensis so as to use compound probiotics more effectively and provide a theoretical basis for the healthy culturing of Trionys sinensis. The probiotics used were compounded in our laboratory and we have applied for a patent( number 201410039520. 3).The probiotic components include bacillus subtilis,bacillus natto,Lactobacillus casei and Candida utilis. Test animals of mean initial weight( 227. 47 ± 14. 42) g were selected for the 140 d experiment and fed a soft-shelled turtle diet three times a day supplemented with different levels of compound probiotics( Group I: 0 mg / kg; Group II:500 mg / kg; Group III: 1 000 mg / kg; Group IV: 2 000 mg / kg). Each group consisted of 1200 individuals and each group was tested in triplicate. The water temperature,food consumption and mortality were recorded each day. After the trial was complete,15 individuals from each group were selected randomly to evaluate digestive enzyme activity in the intestine and 10 individuals for each group were selected and fasted 24 h to measure blood biochemical indices including total protein( TP),total cholesterol( CHO),triglyceride( TG),glucose( GLU),blood urea nitrogen( BUN),globulin( GLB),albumin( ALB),lysozyme( LZM),methane dicarboxylic aldehyde( MDA),superoxide dismutase( SOD),glutathione peroxidase( GSH-PX),glutamic oxalacetic transaminase( AST) and alanine transaminase( ALT). Results indicate that weight gain rate( WGR),specific growth rate( SGR) and survival rate increased significantly and the bait coefficient decreased significantly compared to the control group( P 0. 05). The highest weight gain( 29. 25%),specific growth rate and survival rate as well as the lowest bait coefficient was displayed by Group III. The activities of lipase,amylase,tryptase increased significantly with the addition of compound probiotics( P 0. 05),but the activity of amylase significantly decreased in Group IV with a 2 000 mg / kg compound probiotic supplement. The contents of CHO,BUN and the activity of ALT in serum significantly decreased( P 0. 05),and the level of TG significantly increased( P 0. 05),while the content of TP,GLB,ALB,GLU,MDA and the activity of AST in serum among all experimental groups showed no significant difference( P 0. 05). The activities of LZM,GSH-PX and SOD in serum significantly increased( P 0. 05)in Group Ⅱ and Group Ⅲ,but activities declined in Group IV( P 0. 05). Based on these findings,we conclude that properly compounded probiotic supplementation to the basal diet of Trionyx sinensis can improve growth,increase digestive enzyme activity,enhance nonspecific immunity and antioxidant capacity and decrease the bait coefficient. The appropriate level of compound probiotics added in the basal diet is 1 000- 2 000 mg / kg." @default.
• W2391114897 created "2016-06-24" @default.
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• W2391114897 date "2015-01-01" @default.
• W2391114897 modified "2023-09-23" @default.
• W2391114897 title "Effects of Compound Probiotics on the Growth and Blood Biochemistry of Trionyx sinensis" @default.
• W2391114897 hasPublicationYear "2015" @default.
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