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- W2391115622 abstract "MON88913 is one glyphosate-tolerant genetically modified(GM) cotton event,and which has been approved for using as processed materials since 2007 in China.The aim of this study was to establish event-specific qualitative and quantitative detection methods for MON88913.In this study,the specific primers and TaqMan probes based on the revealed 3' end flanking sequence of GM cotton MON88913 exogenous integration were designed,and the qualitative and quantitative PCR assays were established employing the designed primers and probes.The qualitative PCR assay produced a 231-b Pproduct and the limit of detection(LOD) was 0.05% in 100 ng total cotton genomic DNA,corresponding to about 20 copies haploid cotton genomic DNA.The limit of detection and quantification(LOD and LOQ) of the established quantitative PCR assay were determined to be approximately 10 and 20 copies haploid cotton genomic DNA,respectively.Furthermore,the developed quantitative PCR method was in-house validated by 5 different researchers.In this validation,5 mixed cotton samples with known MON88913 contents were quantified employing the developed quantitative PCR method,and the quantified bias between the true and quantification value from 5 researchers were ranged from 1.59% to 10.12%.All these results suggested that the developed qualitative and quantitative PCR methods were applicable for the identification and quantification of GM cotton MON88913 and its derivates." @default.
- W2391115622 created "2016-06-24" @default.
- W2391115622 creator A5076402120 @default.
- W2391115622 date "2009-01-01" @default.
- W2391115622 modified "2023-09-27" @default.
- W2391115622 title "Event-specific qualitative and quantitative PCR detection methods for genetically modified cotton MON88913" @default.
- W2391115622 hasPublicationYear "2009" @default.
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