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- W2391221821 abstract "Objective:To establish a new simple method for isolating,purifying,and culturing human endometrial glandular epithelial and stromal cells.Methods:Enzymatic digestion,double filtration,and adherence purification were performed to obtain the endometrial glandular epithelial and stromal cells from the endometrial tissues of women undergoing total hysterectomy. These 2 types of cells were observed under light microscope and identified by immunocytochemical staining. Results:The glandular epithelial cells grew in whorls and stained positively for cytokeratin 19(CK19),and the purity was above 92%. The stromal cells were spindle-shaped or polygonal-shaped and stained positively for vimentin,and the purity was above 95%. The numbers of stromal and glandular epithelial cells obtained from 1 endometrium sample were about 10×106 to 25×106 and 4×106 to 6×106,respectively. Conclusion:A better yield of purified human epithelial and stromal cells can be obtained by using this modified culture method." @default.
- W2391221821 created "2016-06-24" @default.
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- W2391221821 date "2007-01-01" @default.
- W2391221821 modified "2023-09-23" @default.
- W2391221821 title "A new method for isolating,purifying,and culturing human endometrial glandular epithelial and stromal cells" @default.
- W2391221821 hasPublicationYear "2007" @default.
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