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- W2391228278 abstract "[Objective] The aim of the study was to screen out the optimum method of extracting maize endosperm DNA.[Method] The improved CTAB extract was used to extract the total genome DNA from maize endosperm with high temperature and normal temperature method.And their extracting effect were compared by the test methods of ultraviolet spectrophotometer,agaroseGel electrophoresis at 0.8% level and PCR.[Result] The concn.and purity of the maize endosperm DNA extracted by high temperature method all accorded with the demand of the optimal molecular biology,while the purity of DNA extracted by normal temperature method didn't accorded with the demand of the optimal molecular.The electrophoresis test showed that there was no degradation in DNA extracted by high temperature method and DNA brands had better uniformity,while the degradation appeared in DNA extracted by normal temperature method.PCR test showed that taking DNA extracted by high temperature method as the templet could get clear PCR bands and taking DNA extracted by normal temperature method as the templet couldn't get clear PCR bands.The optimum concn.of PVPP was ascertained as 6%-10% of maize grain weight.[Conclusion] The high temperature method was more suitable for extracting maize endosperm DNA." @default.
- W2391228278 created "2016-06-24" @default.
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- W2391228278 date "2008-01-01" @default.
- W2391228278 modified "2023-09-26" @default.
- W2391228278 title "Comparison on Two methods of Extracting Maize Endosperm DNA" @default.
- W2391228278 hasPublicationYear "2008" @default.
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