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- W2391233314 abstract "AIM To analyze the differentiation features of embryonic stem cells(ESCs) induced by visceral endoderm-like END-2 cell.METHODS Mouse embryonic fibroblasts(MEF)were used to promote the growth of ESCs and keep them in an undifferentiated state.Day 2-3 embryoid bodies(EBs) were derived from ESCs and then co-cultured with END-2 cells to induce into cardiomyocytes.ENs in group 1 and group 2 were co-cultured respectively with END-2 cells and END-2 cells conditioned medium,those in group 3 were co-cultured with END-2 cell lain by a layer of agar.Spontaneous differentiation group was used as control.The expression of cardiac specific cardiac troponin-T(TnT) was detected by using immunofluoresence and the ultrastructural analysis of ESCs-derived cardiomyocytes was scanned by transmission electron micrograph.RESULTS EBs in all the groups displayed spontaneously beating foci.With the passage of time,the total number of spontaneously beating EBs increased.All the beating cardiomyocytes derived from ESCs expressed cardiac-specific proteins for TnT and the cardiac-specific ultrastructure could be observed.Under the condition of direct contact with END-2 cells,more purified cells could be obtained.CONCLUSION A differentiation-inducing factor secreted by END-2 cells can induce ESCs into cardiomyocytes.Direct contact between END-2 cells and ESCs is not necessarily required for the differentiation but can produce more purified cells." @default.
- W2391233314 created "2016-06-24" @default.
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- W2391233314 date "2006-01-01" @default.
- W2391233314 modified "2023-09-23" @default.
- W2391233314 title "Visceral endoderm-like END-2 cell induces differentiation of mouse embryonic stem cell into cardiomyocytes" @default.
- W2391233314 hasPublicationYear "2006" @default.
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