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- W2391262272 abstract "Objective To explore the influence of bacteriostasis on Enterococcus faecalis by antibacterial substance purified from combination of Cortex Phellodendri and Galla Chinensis by high- speed counter- current chromatography.Methods 150 strains of clinical wild Enterococcus faecalis isolates were selected and randomly divided into Group I, Group II and Group III, 50 strains in each group were made up as 2 000 cfu/mL~3 000 cfu/mL Enterococcus faecalis solution. The combination of Cortex Phellodendri and Galla Chinensis were formulated as solution A of 1 g/mL. Antibacterial substance purified by HSCCC was prepared as solution B with the concentration of 1g/mL. The Enterococcus faecalis solution was smeared on mixed culture medium of M-H medium and solution A in Group I, mixed cultured medium of M-H and solution B in Group II, and merely on M-H in Group III. The above- mentioned culture medium were all pasted with the drug sensitivity paper of ciprofloxacin(CIP), erythrocin(ERY), tetracycline(TE), penicillin(PEN),clindamycin(ClE) and oxacilin(OXA) and clarithmmy cinl(CLA) so as to observe the drug sensitivity of the culture medium. Results The resistance rates of CIP, ERY,TE, PEN, ClE, OXA and CLA in Group I were 26%,40%,36%,30%,42%,38% and 24%, respectively;and those in Group II were 10%,26%,14%,8%,18%,24% and 4%, respectively and in Group III as 76%,92%,100%,88%,96%,90% and 60%.The resistance rates of CIP, ERY, TE, PEN, ClE, OXA and CLA in Group IIIGroup I Group II,and the statistically significant differences of drug resistance rates among the three groups were found(P 0.05).Conclusions The antibacterial substance purified from the combination of Cortex Phellodendri and Galla Chinensis purified by HSCCC has a strong antibacterial activity on clinical Enterococcus faecalis isolates,and its antibacterial action is stronger than the decoction of combination golden cypress and Chinese Gall." @default.
- W2391262272 created "2016-06-24" @default.
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- W2391262272 date "2014-01-01" @default.
- W2391262272 modified "2023-09-25" @default.
- W2391262272 title "Effect of antibacterial substance purified from combination of Cortex Phellodendri and Galla Chinensis by high-speed counter-current chromatography on Enterococcus faecalis" @default.
- W2391262272 hasPublicationYear "2014" @default.
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