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- W2391290932 abstract "Three embryonic stem cell lines, designated MESPU 17, MESPU 18 and MESPU 19 were establishcd from C57 BL/6 J blastocysts. The stem cell pattern of the new lines was demonstra-ted by cytological observations and strong AKP activity. All of the ES lines were XY type karyotype with normai diploid composition of 70%, 88% and 59% respectively. They were able to grow and differcntiate to form simple embryoid bodies in vitro and to form teratocar-cinoma tumors in vivo. Comparing these cells with CCE and MESPU 13 derived from 129/ter, the cells were comparatively large, the rate of growth of the cells was relatively slow in vitro, and the cells were considerably sticky and easier to adherent to microinjection pipet. No chimera of MESPU 17 or MESPU 18 was got by the method of the blastocyst injection of BALB/c or KMW (Kun Ming White), but by the msthod of injection of ES celis of MESPU 17 or MESPU 18 into 8-cell embryos of KMW and co-culture with KMW embryos, the chime-ras had been obtained." @default.
- W2391290932 created "2016-06-24" @default.
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- W2391290932 date "1996-01-01" @default.
- W2391290932 modified "2023-09-26" @default.
- W2391290932 title "ESTABLISHMENT AND CHARACTERISTICS OF ES CELL LINES DERIVED FROM C 57 BL/6 J MICE" @default.
- W2391290932 hasPublicationYear "1996" @default.
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