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- W2391297114 abstract "Ovine cumulus-oocyte complex was matured for 24 hours and fertilised for 17 hours in the vitro culture.Blastocysts rate was compared between ultracentrifugation and the control groups.By applying the method of ultracentrifugation could move the opaque lipid droplets to the edge of the zygotes so that their pronuclears were clear visualization.The breast specific expression of ALR and the non-specific expression of EGFP gene vector DNA were injected into the pronuclear of the fertilized eggs.Blastocysts rate of control were higher than ultracentrifugation group,but no significant difference(P0.05).After microinjection for 2 days the transgenic zygotes expressed green fluorescence.The five positive zygotes were tested by PCR.GFP can be used as a genetic marker to screen transgenic embryos.The establishment of transgenic embryo screening methods using GFP as genetic marker will greatly increase efficiency of gene transfection and the production of transgenic sheep." @default.
- W2391297114 created "2016-06-24" @default.
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- W2391297114 date "2007-01-01" @default.
- W2391297114 modified "2023-09-23" @default.
- W2391297114 title "Transgic Ovine Embryos Production by Means of Pronuclear Microinjection" @default.
- W2391297114 hasPublicationYear "2007" @default.
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