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- W2391301934 abstract "Human ciliary neurotrophic factor(CNTF) is a neurotrophic factor(NTF) not included in NTF family. Recently body weight loss effect of CNTF was discovered in many studies, but clinical application has been limited as immunogenicity. Prepared ciliary neurotrophic factor mutant (CNTFm) with prokaryotic expression and analyzed effect on weight reduction. Chemical synthesis DNA of CNTFm, in which polypeptide is truncated 15aa at C-terminus. The DNA is subcloned in temperature and induced vetor pBV220, then the recombinant plasmid is transformed in E.coli BL21 and recombination protein expression is induced by culture at 42 ℃. The recombination protein is purified with ion exchange chromatography. The normal mice' weight boss tests are used for activity analysis. The expressed level of the CNTFm can reach over 40% of the total bacteria protein and exist in a form of inclusion body. After denaturing, refolding and purifying by Q Sepharose-FF, the purity of final product is more than 95%. Activity analysis indicates that the mutant protein obviously reduces body weight of tested mouse, maximal slip of body weight to reach 31.23% with administration 10 days, but the antibody levels induced by the CNTFm are significantly higher. CNTFm is modified by methyl polyethylene glycol succinimidyl propionate(mPEG-SPA). Compared with unmodified CNTFm, the pEG-CNTFm has 66% biologic activity of unmodified CNTFm in vivo, but it has significantly decreased the immunogenicity." @default.
- W2391301934 created "2016-06-24" @default.
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- W2391301934 date "2009-01-01" @default.
- W2391301934 modified "2023-09-23" @default.
- W2391301934 title "Preparation and PEGylation of Recombinant Human Ciliary Neurotrophic Factor Mutant" @default.
- W2391301934 hasPublicationYear "2009" @default.
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