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- W2391317943 abstract "Objective:To investigate the effect of PNP/MePdR suicide gene system on hepatoma cells. Methods:An eukaryotic expression vector pcDNA3.0/PNP was constructed by inserting PNP gene into pcDNA3.0. Then it was transfected into a hepatoma cell line,HepG2,by liposome-mediation. The HepG2/PNP cell line with stable PNP gene expression was established via G418 selection. The cell growth curves were determined by Trypan blue staining. The sensitivity of HepG2/PNP to MePdR and the bystander effect was measured by MTT and FCM assay, respectively. The enzymatic activity of the product of PNP gene was determined with high pressure liquid chromatography (HPLC). Results:The HepG2/PNP cells were sensitive to MePdR. IC_ 50 value was 4.5 μmol/L after 4-d treatment with MePdR. MePdR 100 μmol/L killed almost all the HepG2/PNP cells. As for the bystander effect, after 8-d treatment with MePdR 100 μmol/L,5% HepG2/PNP cells in mixed culture could induce all cell death. HPLC analysis showed that PNP gene product could convert MePdR into 6-MP. Conclusion:PNP/MePdR suicide gene system has an advantage over traditional suicide gene systems in hepatoma gene therapy. Our study would lay a substantial basis upon hepatoma therapy in vivo ." @default.
- W2391317943 created "2016-06-24" @default.
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- W2391317943 date "2006-01-01" @default.
- W2391317943 modified "2023-09-25" @default.
- W2391317943 title "PNP/MeP-dR suicide gene system killed hepatoma cells" @default.
- W2391317943 hasPublicationYear "2006" @default.
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