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- W2391319573 abstract "Spermatozoa cryopreservation of scallops Patinopecten yessonesis and Chlamys farreri from Chandao and Qngdao in Shandong Province was studied in 1997. Spermatozoa were collected from mta scallops by natural spawning and artificial clissechng. A series of tests were conducted tO develop a suitable freezing and thawing tecboque for the cryopreservation of the scallop spermatozoa.The effects of diluent composihons on spermatozoa survival rate and hme after thawing showed tha naed sea water was the best diluent and the average survival at of thawed spermatozoa was 80%-90% and the survival hme was more than 2h. DMSO was better than glycerin in cryopreservation of spermatozoa of scallops and the spermatozoa survival rate and time were 85%-90% and 2.5-3.5h respechvely. The results of freezing and thawing testS showed tha the survival rate of the spermatozoa could be as high as 80%- 85% when spermatozoa were maintrined at 25cm,20cm or 10cm above LN2 surface for 8-10min. The thewing test showed that the highest survival rate of LN2 frozen-thawed spermatozoa could be obtained when the spermatozoa were maintained at 10cm, 15cm or 20cm above LN2 for 15-30min and then inunrsed in tap warer (7- 11℃) hll melting. The survival rate and hme were 80%- 90% and apprxiInately 3h,respechvely. Fresh eggs could be fertilized by cryopreserved spermatozoa and the fenilization rate and hatohery at were 85%-95% and 70%-85%, respechvely." @default.
- W2391319573 created "2016-06-24" @default.
- W2391319573 creator A5061573962 @default.
- W2391319573 date "1999-01-01" @default.
- W2391319573 modified "2023-09-23" @default.
- W2391319573 title "STUDIES ON SPERMATOZOA CRYOPRESERVATION OF SCALLOPS PATINOPECTEN YESSONESIS AND CHLAMYS FARRERI" @default.
- W2391319573 hasPublicationYear "1999" @default.
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