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- W2391339706 abstract "BHK21 cell is a susceptible host for a lot of animal viruses and a commonly used cell line for virus vaccines production.In order to study the replication disciplinarian of influenza A virus in the line of BHK21,the SYBR Green Ⅰreal-time RT-PCR(RRT-PCR) based on M gene is developed to study the replication disciplinarian of influenza A virus in the line of BHK21.The result shows that cytopathic effect(CPE) is observed at 24 h post infection(PI) when inoculation 1 MOI influenza A virus gets into BHK21 cells,and a large number of cells fall off and the desegregation is at 36 h PI.The virus load is 1.77×106copies/mL at 6 h,2.39×106copies/mL at 12 h,6.58×105copies/mL at 24 h and 2.87×105copies/mL at 36 h PI.The results in this study indicate that the RRT-PCR provides an accurate quantification of influenza A virus.This study also provides basic information for the interaction between cells and virus and vaccine production of influenza A virus." @default.
- W2391339706 created "2016-06-24" @default.
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- W2391339706 date "2012-01-01" @default.
- W2391339706 modified "2023-09-23" @default.
- W2391339706 title "Study on the proliferation profile of influenza A virus in BHK21 cells" @default.
- W2391339706 hasPublicationYear "2012" @default.
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