Matches in SemOpenAlex for { <https://semopenalex.org/work/W2391341443> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2391341443 abstract "OBJECTIVE:To explore the blocking effect of siRNA on the expression of Survivin in gene in osteosarcoma cell line MG63.METHODS:According to Survivin cDNA coding sequence,the specific RNA interference(RNAi)fragments targeting Survivin gene were designed and synthesized,which were cloned into pSilencer 3.0-H1 neo plasmid vector,and the shRNA eukaryotic expression vector siRNA Survivin targeting Survivin gene was constructed.After the vector was constructed,MG63 cells were transfected with negative control vector siRNA neg or RNAi vectors and selected by G418.Expression of mRNA and protein of Survivin in the stable transfected cells was investigated seperately by RT-PCR,Western blot,immunofluorescence microscopy,and flow cytometry.RESULTS:The specific siRNA eukaryotic expression vector PsvA and PsvB targeting Survivin gene were constructed successfully.The stable transfectants containing negative control vector siRNA neg,PsvA and PsvB were obtained.Expression of mRNA and protein of Survivin was inhibited significantly in MG63/PsvB cells.Whereas Survivin gene expression levels were hardly changed in the other groups.Otherwise,MG63/PsvB had significant decreases in cell number compared with other transfectants.Analysis of DNA content in PsvB transfectants revealed a 7-fold increase in the fraction of sub-G1 peak(apoptotic peak)as compared with vector control and PsvA transfectants,under the same experimental conditions,P0.01.CONCUSION:Survivin gene expression can be suppressed markedly by specific shRNA in MG63 cells,which establishes the experimental foundation for further studying the biological functions and its mechanisms of Survivin in MG63 cells." @default.
- W2391341443 created "2016-06-24" @default.
- W2391341443 creator A5086640695 @default.
- W2391341443 date "2007-01-01" @default.
- W2391341443 modified "2023-09-27" @default.
- W2391341443 title "Inhibition of Survivin gene expression in human osteosarcoma cell line MG63" @default.
- W2391341443 hasPublicationYear "2007" @default.
- W2391341443 type Work @default.
- W2391341443 sameAs 2391341443 @default.
- W2391341443 citedByCount "0" @default.
- W2391341443 crossrefType "journal-article" @default.
- W2391341443 hasAuthorship W2391341443A5086640695 @default.
- W2391341443 hasConcept C104317684 @default.
- W2391341443 hasConcept C150194340 @default.
- W2391341443 hasConcept C153911025 @default.
- W2391341443 hasConcept C166703698 @default.
- W2391341443 hasConcept C190232843 @default.
- W2391341443 hasConcept C22615655 @default.
- W2391341443 hasConcept C2775975398 @default.
- W2391341443 hasConcept C33161422 @default.
- W2391341443 hasConcept C40767141 @default.
- W2391341443 hasConcept C54009773 @default.
- W2391341443 hasConcept C54355233 @default.
- W2391341443 hasConcept C67705224 @default.
- W2391341443 hasConcept C81885089 @default.
- W2391341443 hasConcept C86803240 @default.
- W2391341443 hasConceptScore W2391341443C104317684 @default.
- W2391341443 hasConceptScore W2391341443C150194340 @default.
- W2391341443 hasConceptScore W2391341443C153911025 @default.
- W2391341443 hasConceptScore W2391341443C166703698 @default.
- W2391341443 hasConceptScore W2391341443C190232843 @default.
- W2391341443 hasConceptScore W2391341443C22615655 @default.
- W2391341443 hasConceptScore W2391341443C2775975398 @default.
- W2391341443 hasConceptScore W2391341443C33161422 @default.
- W2391341443 hasConceptScore W2391341443C40767141 @default.
- W2391341443 hasConceptScore W2391341443C54009773 @default.
- W2391341443 hasConceptScore W2391341443C54355233 @default.
- W2391341443 hasConceptScore W2391341443C67705224 @default.
- W2391341443 hasConceptScore W2391341443C81885089 @default.
- W2391341443 hasConceptScore W2391341443C86803240 @default.
- W2391341443 hasLocation W23913414431 @default.
- W2391341443 hasOpenAccess W2391341443 @default.
- W2391341443 hasPrimaryLocation W23913414431 @default.
- W2391341443 hasRelatedWork W1533067014 @default.
- W2391341443 hasRelatedWork W1563568789 @default.
- W2391341443 hasRelatedWork W2090776230 @default.
- W2391341443 hasRelatedWork W2354618606 @default.
- W2391341443 hasRelatedWork W2355145806 @default.
- W2391341443 hasRelatedWork W2356985188 @default.
- W2391341443 hasRelatedWork W2359324396 @default.
- W2391341443 hasRelatedWork W2372033869 @default.
- W2391341443 hasRelatedWork W2375863370 @default.
- W2391341443 hasRelatedWork W2378542958 @default.
- W2391341443 hasRelatedWork W2378564201 @default.
- W2391341443 hasRelatedWork W2380288995 @default.
- W2391341443 hasRelatedWork W2381709296 @default.
- W2391341443 hasRelatedWork W2383677872 @default.
- W2391341443 hasRelatedWork W2385778052 @default.
- W2391341443 hasRelatedWork W2388386957 @default.
- W2391341443 hasRelatedWork W2389245519 @default.
- W2391341443 hasRelatedWork W2390997656 @default.
- W2391341443 hasRelatedWork W2413593289 @default.
- W2391341443 hasRelatedWork W3031522577 @default.
- W2391341443 isParatext "false" @default.
- W2391341443 isRetracted "false" @default.
- W2391341443 magId "2391341443" @default.
- W2391341443 workType "article" @default.