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- W2391351276 abstract "Objective:To investigate the treatment effect of recombinant plasmid pGPU6/GFP/Neo-hTERT-shRNA targeting hTERT gene on human colorectal cancer SW480 cell xenograft in nude mice.Methods: Human colorectal cancer SW480 cells were subcutaneously implanted under the skin of the right armpit to establish nude mice model of colorectal cancer,after the tumors grew to a definite size.The mice were randomly divided into three groups: normal saline(NS group),pGPU6/GFP/Neo-NC-shRNA group(NC-shRNA group)and pGPU6/GFP/Neo-hTERT-shRNA group(hTERT-shRNA group).After each group was treated for 6 consecutive times,the growth status of the tumor was observed,tumor volume was measured,tumor growth curve was drawn,tumor tissue morphology was observed with H-E staining,the expression of hTERT protein in the tumors was detected by immunohistochemistry,cell apoptosis was inspected by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling(TUNEL),and the expression of hTERT mRNA was checked by RT-PCR.Results: The growth of tumor volume became slower in hTERT-shRNA group than did that in NS group and NC-shRNA group.Compared with NS group and NC-shRNA group,the tumor cell morphology changed obviously and the number of apoptotic cells increased significantly in the transplanted tumor tissues in hTERT-shRNA group([36.3±5.05]% vs [5.25±1.06]%,[6.95±1.07]%,P0.01).Compared with NS group and NC-shRNA group,the expression of hTERT protein was significantly inhibited in hTERT-shRNA group([171.42±30.94] vs [146.89±21.43],[137.35±25.49],P0.01).Compared with NS group and NC-shRNA group,the expression of hTERT mRNA was significantly inhibited in hTERT-shRNA group(0.39±0.09 vs 0.81±0.34,0.75±0.21,P0.05).Conclusion: Recombinant plasmid pGPU6/GFP/Neo-hTERT-shRNA promotes apoptosis of implanted human colorectal cancer by down-regulating the expression of hTERT mRNA and hTERT protein in tumor tissues,thus inhibiting the growth." @default.
- W2391351276 created "2016-06-24" @default.
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- W2391351276 date "2012-01-01" @default.
- W2391351276 modified "2023-09-23" @default.
- W2391351276 title "Inhibition of pGPU6/GFP/Neo-hTERT-shRNA on colorectal cancer SW480 cell xenograft in nude mice" @default.
- W2391351276 hasPublicationYear "2012" @default.
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