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- W2391451401 abstract "The specificities of the primers and TaqMan probe that were designed according to the hlyA gene of Listeria monocytogenes were tested.The real-time PCR assay was developed by amplifying quatitatively hlyA gene and constructing standard curve of positive recombinant plasmids.Then the assay was applied in artificially inoculated meat.The results showed that all of the strains of Listeria monocytogenes(n=10) tested were positive and all other nonspecific strains(n=6) tested were negative.The correlation coefficient of the standard curve was 0.998 and the sensitivity was 19.5 copies.μL-1 in positive recombinant plasmids.Then the detection limit of 2.8× 102 cfu.g-1 was determined in artificially inoculated meat.The assay was specific and sensitive highly,which would be extended to detect quatitatively Listeria monocytogenes in meat." @default.
- W2391451401 created "2016-06-24" @default.
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- W2391451401 date "2012-01-01" @default.
- W2391451401 modified "2023-09-28" @default.
- W2391451401 title "Real-time TaqMan PCR for detection of Listeria monocytogenes in meat" @default.
- W2391451401 hasPublicationYear "2012" @default.
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