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- W2391477992 abstract "An amidase from a strain Bacillus cereus ZJB-07112 was purified to homogeneity by using sonication, anion-exchange chromatography, phenyl-sepharose chromatography.The molecular weight of amidase was estimated to be 60.6×103 by 12.5% SDS-PAGE.Its N-terminal sequence was ATIRPDDKAI.The optimum pH and temperature of the amidase for acrylamide were pH 7.5 and 35℃, respectively.The enzyme was unstable at a temperature over 50℃ and only 10.8% activity was retained after exposure to 60℃ for 30 min.Most of metal ions and EDTA had no significant effect on the enzyme activity, whereas Hg+,Ag+ and urea caused obvious inhibition.The Km and Vmax values of the amidase for acrylamide were 2.64 mmol·L-1 and 0.6 μmol·min-1·ml-1, respectively." @default.
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- W2391477992 date "2008-01-01" @default.
- W2391477992 modified "2023-09-24" @default.
- W2391477992 title "Purification and characterization of amidase from Bacillus cereus ZJB-07112" @default.
- W2391477992 hasPublicationYear "2008" @default.
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