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- W2391479029 abstract "Objective To obtain a recombinant adeno-associated virus (AAV) vector expressing mouse repressor of GATA-3(ROG) including the preparation. Methods The total RNA was extracted from cultured CTLL-2 cells. The gene sequence coding ROG was amplified by one-step reverse transcription-polymerase chain reaction(RT-PCR). The fragment was inserted into AAV (pAAV-MCS) ultimatly after two cloning vectors to obtain the recombinant pAAV-ROG; The Hela cells were cotransfected with pAAV-RC and pHelper. Results The ROG cDNA by RT-PCR was amplified from the total RNA extracted from cultured CTLL-2 cells. The resultant fragment was cloned into pAAV confirmed by restriction digestion and sequencing; high titer recombinative virus was obtained through cotransfecting the Hela cells. Conclusions The mouse repressor of GATA-3 gene recombinated with AAV expressing plasmid lays down the foundation for gene therapy of bronchchial asthma and other allergic diseases." @default.
- W2391479029 created "2016-06-24" @default.
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- W2391479029 date "2004-01-01" @default.
- W2391479029 modified "2023-09-26" @default.
- W2391479029 title "Construction of recombinant adeno-associated virus vector of highly expressed transcription factor in hibitor, ROG" @default.
- W2391479029 hasPublicationYear "2004" @default.
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