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- W2391522830 abstract "Objective:To investigate the effect of ataxia telangiectasia mutated gene(ATM gene) in the adaptive response(AR) induced by low dose ionizing radiation in EL-4 cells.Methods:The ATM mRNA levels and protein expression of EL-4 cells were detected by reverse transcription polymerase chain reaction(RT-PCR) and immumofluorescence method after the cells received the pre-exposure of 0.075 Gy X-rays(inductive dose,D1) and the succeeding high dose irradiation(challenge dose,D2;1.0,1.5,and 2.0 Gy X-rays,respectively) with wortmannin(inhibitor of ATM).The apoptosis and cell cycle progression of EL-4 cells were detected with flow cytometry.Results:The expression of ATM mRNA and protein could be intercepted by wortmannin.Treatment with wortmannin in EL-4 cells,and then challenged by high doses irradiation directly or received pre-exposure of low inductive dose and the succeeding challenge dose irradiation could induced the AR of EL-4 cells.Conclusion:The AR induced by low dose ionizing radiation may be mediated without ATM gene in EL-4 cells." @default.
- W2391522830 created "2016-06-24" @default.
- W2391522830 creator A5002118757 @default.
- W2391522830 date "2012-01-01" @default.
- W2391522830 modified "2023-09-23" @default.
- W2391522830 title "Effect of ATM gene in the adaptive response of EL-4 cells induced by low dose ionizing radiation" @default.
- W2391522830 hasPublicationYear "2012" @default.
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