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- W2391528090 abstract "Objective:To develop a safe cell model based on the enhanced green fluorescent protein(EGFP) for anti-HIV drug evaluation.Methods:The infectious HIV-1 clone pUC18-HIV-NL4-3 was reconstructed to be the pseudovirus plasmid pUC18-NL4-3-EGFP,in which the EGFP gene replaced the HIV-1 env gene.The human embryonic kidney cell line 293FT was transfected with pUC18-NL4-3-EGFP and vesicular stomatitis virus glycoprotein(VSV-G) producing plasmids.The reporter gene expression in the co-transfected cells was examined by detecting green fluorescence and the conditioned medium containing the pseudovirus was used to infect naive 293FT cells.To assess whether this pseudovirus production and infection system can be used as an anti-HIV drug evaluation cell model or not,the AIDS drug AZT(Zidovudine) was applied into the cell culture at different concentration,and the inhibitory effect of AZT on pseudovirus infection was analyzed via detecting EGFP expression with fluorescence microscope and fluorescent activated cell sorter.Results:The pseudovirus plasmids and VSV-G expressing plasmids co-transfected 293FT cells and the conditioned medium infected naive 293FT cells both efficiently produced EGFP.AZT inhibited the EGFP expression in the conditioned medium infected 293FT cells in a dose dependant manner.Conclusion:A safe system based on EGFP expression and detection and representing HIV pseudovirus replication and secretion was successfully established,which has the potential to be used for anti-HIV drug evaluation." @default.
- W2391528090 created "2016-06-24" @default.
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- W2391528090 date "2008-01-01" @default.
- W2391528090 modified "2023-09-28" @default.
- W2391528090 title "A Safe Anti-HIV Drug Evaluation System Based on EGFP Expression" @default.
- W2391528090 hasPublicationYear "2008" @default.
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