Matches in SemOpenAlex for { <https://semopenalex.org/work/W2391531199> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2391531199 abstract "Objective:To investigate the role of AREG in PGE2-induced proliferation of cholangiocarcinoma cells(CCLP1 cells).Methods:CCLP1 cells were treated with PGE2,EP1-4 receptor agonist,EP2 receptor antagonist H89,PKA inhibitor H89,cAMP inhibitor SQ22536.Western blot and WST were used to examine the expression of AREG protein and the cell proliferation ability in CCLP1 cells.Results:The level of AREG protein increased by 44.2%(P 0.05) after treated with 10 μmol/L PGE2.It was previously proved that PGE2 could promote the proliferation of CCLP1 cells,however,this effect was obviously inhibited by 20%(P 0.01) when CCLP1 cells were pretreated with the 20 μmol/L neutralizing-antibody(Mab262 and SAB1402118) of AREG.Then four selective EP receptor agonists(17-phenyltrinor Prostaglandin E2,Butaprost,Sulprostone and Prostaglandin E1 Alcohol) 10 μmol/L were used to stimulate CCLP1 cells,it were found that only EP2 agonist could induce the expression of AREG protein,increased by 20.1%(P 0.05),whereas the other three agonists downregulated the protein expression of AREG.In agreement with this result,the expression of AREG protein improved by EP2 agonist was indeed suppressed by 20%,when CCLP1 cells were pretreated with 10 μmol/L AH6809,the EP2 antagonist.After treated with EP2 receptor agonist(10 μmol/L) for 24 h,the proliferation ability of CCLP1 cells increased by 26.5%.PKA inhibitor H89 and cAMP inhibitor SQ22536 can restrict the AREG protein increase induced by PGE2,and the AREG protein expression was downregulated by 54.4% and 46.6% respectively,compared with PGE2 treated group.It was reported that PGE2 could induce the phosphorylation of CREB in CCLP1 cells,which could bind and activate the CRE element of AREG promoter.However,H89 and SQ22536 could strikingly inhibit the phosphorylation of CREB induced by PGE2.At the same time,CMV500-DN-CREB plasmid was transfected into CCLP1 cells,resulting in a striking restriction of AREG protein expression induced by PGE2.Conclusion:Prostaglandin E2 might promote the cell proliferation of CCLP1 cells through EP2 receptor,which consequently upregulates the expression of AREG by activation of cAMP-PKA-CREB pathway." @default.
- W2391531199 created "2016-06-24" @default.
- W2391531199 creator A5074051847 @default.
- W2391531199 date "2012-01-01" @default.
- W2391531199 modified "2023-09-24" @default.
- W2391531199 title "PGE_2 promotes the proliferation of cholangiocellular carcinoma cells by up-regulating AREG protein expression" @default.
- W2391531199 hasPublicationYear "2012" @default.
- W2391531199 type Work @default.
- W2391531199 sameAs 2391531199 @default.
- W2391531199 citedByCount "0" @default.
- W2391531199 crossrefType "journal-article" @default.
- W2391531199 hasAuthorship W2391531199A5074051847 @default.
- W2391531199 hasConcept C104317684 @default.
- W2391531199 hasConcept C126322002 @default.
- W2391531199 hasConcept C134018914 @default.
- W2391531199 hasConcept C153911025 @default.
- W2391531199 hasConcept C170493617 @default.
- W2391531199 hasConcept C178592051 @default.
- W2391531199 hasConcept C185592680 @default.
- W2391531199 hasConcept C2776415932 @default.
- W2391531199 hasConcept C2777956040 @default.
- W2391531199 hasConcept C2778938600 @default.
- W2391531199 hasConcept C55493867 @default.
- W2391531199 hasConcept C62478195 @default.
- W2391531199 hasConcept C71924100 @default.
- W2391531199 hasConcept C86803240 @default.
- W2391531199 hasConceptScore W2391531199C104317684 @default.
- W2391531199 hasConceptScore W2391531199C126322002 @default.
- W2391531199 hasConceptScore W2391531199C134018914 @default.
- W2391531199 hasConceptScore W2391531199C153911025 @default.
- W2391531199 hasConceptScore W2391531199C170493617 @default.
- W2391531199 hasConceptScore W2391531199C178592051 @default.
- W2391531199 hasConceptScore W2391531199C185592680 @default.
- W2391531199 hasConceptScore W2391531199C2776415932 @default.
- W2391531199 hasConceptScore W2391531199C2777956040 @default.
- W2391531199 hasConceptScore W2391531199C2778938600 @default.
- W2391531199 hasConceptScore W2391531199C55493867 @default.
- W2391531199 hasConceptScore W2391531199C62478195 @default.
- W2391531199 hasConceptScore W2391531199C71924100 @default.
- W2391531199 hasConceptScore W2391531199C86803240 @default.
- W2391531199 hasLocation W23915311991 @default.
- W2391531199 hasOpenAccess W2391531199 @default.
- W2391531199 hasPrimaryLocation W23915311991 @default.
- W2391531199 hasRelatedWork W1487471976 @default.
- W2391531199 hasRelatedWork W1580212652 @default.
- W2391531199 hasRelatedWork W2003101395 @default.
- W2391531199 hasRelatedWork W2009421944 @default.
- W2391531199 hasRelatedWork W2012668469 @default.
- W2391531199 hasRelatedWork W2051184078 @default.
- W2391531199 hasRelatedWork W2063005063 @default.
- W2391531199 hasRelatedWork W2114224769 @default.
- W2391531199 hasRelatedWork W2134953849 @default.
- W2391531199 hasRelatedWork W2347854487 @default.
- W2391531199 hasRelatedWork W2351054891 @default.
- W2391531199 hasRelatedWork W2356994458 @default.
- W2391531199 hasRelatedWork W2365796677 @default.
- W2391531199 hasRelatedWork W2368140489 @default.
- W2391531199 hasRelatedWork W2380947081 @default.
- W2391531199 hasRelatedWork W2384998178 @default.
- W2391531199 hasRelatedWork W2388792820 @default.
- W2391531199 hasRelatedWork W2418209225 @default.
- W2391531199 hasRelatedWork W2999833101 @default.
- W2391531199 hasRelatedWork W40801990 @default.
- W2391531199 isParatext "false" @default.
- W2391531199 isRetracted "false" @default.
- W2391531199 magId "2391531199" @default.
- W2391531199 workType "article" @default.