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- W2391565246 abstract "AIM: To investigate the effect of yigu capsule drug-containing serum on proliferation, ALP activity and expression of interleukin-11 mRNA in Sprague-Dawley rat osteobalsts in the co-culture system. METHODS: (1) Osteoblasts and osteoclasts were isolated from 1 and 5-day-old Sprague-Dawley rats, respectively. The osteoblasts-osteoclasts co-culture system was built to prevent the two kinds of cells from contact and allow the media to exchange. The experiment included two groups, drug-containing sera group and control group. (2) The osteoblasts proliferation, ALP activity and expression of interleukin-11 mRNA were detected by the MTT, 4-aminoantipyrine spectrometric methods and FQ-PCR,respectively. RESULTS: In drug-containing sera group, the osteoblasts proliferation, ALP activity, expression of IL-11 mRNA were higher than those of control group (P0.05). CONCLUSION: Rat sera containing yigu capsule can obviously enhance the osteoblast proliferation, ALP activity and expression of IL-11 mRNA." @default.
- W2391565246 created "2016-06-24" @default.
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- W2391565246 date "2006-01-01" @default.
- W2391565246 modified "2023-09-26" @default.
- W2391565246 title "Effect of yigu capsule drug-containing serum on proliferation, ALP activity and expression of interleukin-11 mRNA in rat osteoblasts in the co-culture" @default.
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