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- W2391565327 abstract "The breeding of crops depends on a lot of germplasm resources.Many hybrid plants were generated from inter-specific and inter-generic crossing.It is a new technology to expand germplasm resource of crops.In this paper the culture factors of fusion-protoplasts between Brassica napus L.and Matthiola incana B were analyzed.The appropriate condition of fusion-protoplasts culture were that: the fusion-protoplasts were cultured in improved KM8p medium supplemented with 1.5 mg/L 2,4-D,0.5 mg/L NAA and 0.5 ml/L BA with the method of Thin Liquid Layer.0.3 mol/L sucrose in combination with 0.1 mol/L glucose that was used as osmotic regulator were found superior to other combinations.The fusion-protoplast started to be divided after 3~6 days,and the division frequency was 13.8 % after being cultured for 7 days.The cell clone and small callus could be obtained in 35 days and the plating efficiency was 6.5 %." @default.
- W2391565327 created "2016-06-24" @default.
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- W2391565327 date "2006-01-01" @default.
- W2391565327 modified "2023-10-08" @default.
- W2391565327 title "Factor Affecting the Protoplast Fusion Culture between Brassica napus L.and Matthiola incana B." @default.
- W2391565327 hasPublicationYear "2006" @default.
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