Matches in SemOpenAlex for { <https://semopenalex.org/work/W2391566454> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2391566454 endingPage "18" @default.
- W2391566454 startingPage "14" @default.
- W2391566454 abstract "The encoding region of green fluorescent protein(GFP) gene and the promoter region of S.cerevisiae metallothionein(CUP1) gene were amplified to construct a recombinant expression vector pCUP9K-GFP.By using lithium chloride transformation method,the recombinant expression vector was transformed into P.pastoris GS115 cells to create a yeast strain.Green fluorescent protein can be detected in engineered yeast cells and in their fermentation supernatant,indicating that engineered yeast cells could express GFP normally and then secrete GFP to the extracellular medium.The relative fluorescent intensity of the culture supernatant increased in a concentration-dependent manner after engineered yeast exposed to copper ions in a concentration ranging from 5μmol/L to 1mmol/L.However,the fluorescence of engineered yeast did not respond to chromium,cadmium and arsenic ions.This results suggest that CUP1 promoter can be induced specifically by copper ions,which may be used for the monitoring of environmental copper contamination." @default.
- W2391566454 created "2016-06-24" @default.
- W2391566454 creator A5017934440 @default.
- W2391566454 creator A5032057886 @default.
- W2391566454 creator A5064797445 @default.
- W2391566454 creator A5065518693 @default.
- W2391566454 date "2012-10-25" @default.
- W2391566454 modified "2023-09-23" @default.
- W2391566454 title "Construction of an Engineered Yeast with Green Fluorescent Protein Gene and Its Fluorescence in Response to Copper Ion" @default.
- W2391566454 hasPublicationYear "2012" @default.
- W2391566454 type Work @default.
- W2391566454 sameAs 2391566454 @default.
- W2391566454 citedByCount "0" @default.
- W2391566454 crossrefType "journal-article" @default.
- W2391566454 hasAuthorship W2391566454A5017934440 @default.
- W2391566454 hasAuthorship W2391566454A5032057886 @default.
- W2391566454 hasAuthorship W2391566454A5064797445 @default.
- W2391566454 hasAuthorship W2391566454A5065518693 @default.
- W2391566454 hasConcept C104317684 @default.
- W2391566454 hasConcept C121332964 @default.
- W2391566454 hasConcept C142613039 @default.
- W2391566454 hasConcept C153911025 @default.
- W2391566454 hasConcept C185592680 @default.
- W2391566454 hasConcept C204241405 @default.
- W2391566454 hasConcept C20719415 @default.
- W2391566454 hasConcept C2777576037 @default.
- W2391566454 hasConcept C2779222958 @default.
- W2391566454 hasConcept C40767141 @default.
- W2391566454 hasConcept C55493867 @default.
- W2391566454 hasConcept C62520636 @default.
- W2391566454 hasConcept C86803240 @default.
- W2391566454 hasConcept C91881484 @default.
- W2391566454 hasConceptScore W2391566454C104317684 @default.
- W2391566454 hasConceptScore W2391566454C121332964 @default.
- W2391566454 hasConceptScore W2391566454C142613039 @default.
- W2391566454 hasConceptScore W2391566454C153911025 @default.
- W2391566454 hasConceptScore W2391566454C185592680 @default.
- W2391566454 hasConceptScore W2391566454C204241405 @default.
- W2391566454 hasConceptScore W2391566454C20719415 @default.
- W2391566454 hasConceptScore W2391566454C2777576037 @default.
- W2391566454 hasConceptScore W2391566454C2779222958 @default.
- W2391566454 hasConceptScore W2391566454C40767141 @default.
- W2391566454 hasConceptScore W2391566454C55493867 @default.
- W2391566454 hasConceptScore W2391566454C62520636 @default.
- W2391566454 hasConceptScore W2391566454C86803240 @default.
- W2391566454 hasConceptScore W2391566454C91881484 @default.
- W2391566454 hasIssue "10" @default.
- W2391566454 hasLocation W23915664541 @default.
- W2391566454 hasOpenAccess W2391566454 @default.
- W2391566454 hasPrimaryLocation W23915664541 @default.
- W2391566454 hasRelatedWork W1520484238 @default.
- W2391566454 hasRelatedWork W165008294 @default.
- W2391566454 hasRelatedWork W1970078207 @default.
- W2391566454 hasRelatedWork W1976485510 @default.
- W2391566454 hasRelatedWork W1995846306 @default.
- W2391566454 hasRelatedWork W2001261701 @default.
- W2391566454 hasRelatedWork W2046633980 @default.
- W2391566454 hasRelatedWork W2055649671 @default.
- W2391566454 hasRelatedWork W2061068107 @default.
- W2391566454 hasRelatedWork W2140824698 @default.
- W2391566454 hasRelatedWork W2374608045 @default.
- W2391566454 hasRelatedWork W2390303927 @default.
- W2391566454 hasRelatedWork W2416143773 @default.
- W2391566454 hasRelatedWork W2418300841 @default.
- W2391566454 hasRelatedWork W3071456953 @default.
- W2391566454 hasRelatedWork W602926398 @default.
- W2391566454 hasRelatedWork W92660892 @default.
- W2391566454 hasRelatedWork W1493122269 @default.
- W2391566454 hasRelatedWork W2107560969 @default.
- W2391566454 hasRelatedWork W2754332824 @default.
- W2391566454 hasVolume "32" @default.
- W2391566454 isParatext "false" @default.
- W2391566454 isRetracted "false" @default.
- W2391566454 magId "2391566454" @default.
- W2391566454 workType "article" @default.