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- W2391567239 abstract "Objective In order to research the function of GVDE gene which was cloned from ginger leaves.Method Using tobacco plants as material,an antisense expression GVDE vector to transform tobacco leaves was constructed. Transgenic assays were performed using PCR and PCR-Southern. At the same time the changes of chlorophyll fluorescence parameters and antioxidative enzymes were measured.Result PCR amplification and PCR-Southern demonstrated that this antisense gene was successfully integrated into tobacco genome. NPQ,Fv/Fm in transgenic tobacco were lower than in CK under high light,and the activities of APX,SOD and POD in transgenic tobacco were also lower than in CK.ConclusionThe ability of radiationless energy dissipation decreased evidently in transgenic tobacco under high light." @default.
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- W2391567239 date "2008-01-01" @default.
- W2391567239 modified "2023-09-23" @default.
- W2391567239 title "Changes of Chlorophyll Fluorescence Parameters and Antioxidative Enzymes Activities in Antisense GVDE Tobacco" @default.
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