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- W2391567375 abstract "The multiple infecting pathogen were detected in a sample of yellow mosaic broad bean from Lishui,Zhejiang province,by transmission electron microscopy,ELISA and RT-PCR.The results showed that two kinds of virus were found in leaf sap negative stained with 2%PTA.One was filamentous virus particles with 73% lengths ranging from 600nm to 800nm.The other was spherical virus particles,about 26nm in average diameter.The pathological study revealed that the Ⅱtype cylindrical inclusions and the amorphous inclusions were appeared in cytoplasm of the infected cells,which is the pathological symptom of BYMV infecting.Mitochondrial aggregation and membranous proliferation were also found in the infected cells,which is the pathological symptom of BBWV 2 infecting.One of the viruses were positive reacted with BBWV 2 monoclonal antibody by ELISA experiment.There was 1.7kb special production in 1% gel electrophoresis after RT-PCR using universal primer of genus Potyvirus.Based on all the results above,suggested that the spherical virus were Broad bean wilt virus 2(BBWV 2) and the filamentous virus were Bean yellow mosaic virus(BYMV).They were multiple infecting." @default.
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- W2391567375 date "2006-01-01" @default.
- W2391567375 modified "2023-09-25" @default.
- W2391567375 title "Diagnosis of the multiple pathogen of broad bean yellow mosaic disease" @default.
- W2391567375 hasPublicationYear "2006" @default.
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