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- W2391569339 abstract "Gene aveR is the only putative regulatory gene in avermectin biosynthetic gene cluster from Streptomyces avermitilis NRRL8165.In order to test if aveR is involved in the regulation of transcription of avermectin biosynthetic genes,plasmid pJTU2530 was constructed and introduced into S.avermitilis by conjugation.The aveR mutant ZD10,in which a 1320 bp internal fragment of aveR is replaced by aac(3)IV,was obtained and confirmed through PCR amplification.Analyzed by LC-MS,avermectin productivity was abolished,whereas the yield of oligomycin significantly increased in ZD10.The reverse transcription PCR analysis indicates that the transcription level of aveA3,an essential PKS gene,is drastically reduced in ZD10.Therefore,AveR is proved as a positive regulator of avermectin biosynthesis." @default.
- W2391569339 created "2016-06-24" @default.
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- W2391569339 date "2008-01-01" @default.
- W2391569339 modified "2023-09-26" @default.
- W2391569339 title "Positive Regulation of Avermectin Biosynthesis by AveR in Streptomyces avermitilis" @default.
- W2391569339 hasPublicationYear "2008" @default.
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