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- W2391579935 abstract "The complete helper component-proteinase(HC-Pro) gene was cloned by RT-PCR from diseased maize leaves infected by Henan isolate of Sugarcane mosaic virus(SCMV).The cDNA of the HC-Pro contains a 1 380 bp open reading frame(ORF) and encodes 460 amino acids.Sequence alignment showed that the deduced amino acid sequence of the HC-Pro shared 97% and 98% similarities to homologues from SCMV Beijing isolate and Shandong isolate respectively.However,the Hc-Pro cloned by our group only shared 98% similarity to that of SCMV Henan isolate deposited in the Genbank,and this might indicate that the SCMV Henan isolate has undergone some genetic divesities.A 582 bp long fragment of the HC-Pro was selected to construct the binary expression vector pRNAi-HC-Pro(±) with hairpin RNA structure,and the vector was successfully transformed into the agrobacterium strain LBA4404." @default.
- W2391579935 created "2016-06-24" @default.
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- W2391579935 date "2010-01-01" @default.
- W2391579935 modified "2023-09-26" @default.
- W2391579935 title "Sequence analysis and construction of RNA silencing expression vector of the Hc-Pro gene from Sugarcane mosaic virus-Henan isolate" @default.
- W2391579935 hasPublicationYear "2010" @default.
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