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- W2391633459 abstract "[Objective] The aim was to clone and express the pilA gene of outer membrane protein of Haemophilus parasuis.[Method] The published pilA gene sequence of HPS was analyzed for primer synthesis,and the genome of serotype 5-type of HPS was used as template for PCR amplification of the pilA gene of HPS;the recombinant expression plasmid was constructed and transformed into E.coli BL21(DE3)after induced by IPTG.SDS-PAGE and Western blot analysis were then carried out.[Result] The molecular weight of expressed protein was consistent with the expected(43 kD).[Conclusion] The results provided a foundation for the preparation of subunit vaccine and diagnostic reagents." @default.
- W2391633459 created "2016-06-24" @default.
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- W2391633459 date "2011-01-01" @default.
- W2391633459 modified "2023-09-28" @default.
- W2391633459 title "Cloning and Expression of pilA Gene of Outer Membrane Protein of Haemophilus parasuis" @default.
- W2391633459 hasPublicationYear "2011" @default.
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