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- W2391640277 abstract "Objective: The recombinant plasmid pUDKH, encoding human hepatocyte growth factor(HGF), is potential for gene therapy of peripheral arterial occlusion disease. Establishment and quality control of E.coli cell bank transformed recombinant plasmid pUDKH are critical steps in plasmid pUDKH manufacture. Methods: The recombinant pUDKH was transformed into E.coli DH5α strain, selected clones were screened for high plasmid production and then amplified to generate a Master Cell Bank. One vial from Master Cell Bank was amplified to produce the Working Cell Bank. Both cell banks were subjected to examination with morphology, antibiotic resistance, genetic stability, copy number and biochemical properties. Results and Conclusion: The bank strains could be used for manufacture of plasmid pUDKH with higher production ability and genetic stability after 40 generational culture, but absence of contaminating bacteria, viruses and other adventitious agent." @default.
- W2391640277 created "2016-06-24" @default.
- W2391640277 creator A5086795138 @default.
- W2391640277 date "2006-01-01" @default.
- W2391640277 modified "2023-09-23" @default.
- W2391640277 title "Establishment and Quality Control of Escherichia coli Cell Bank with Recombinant Plasmid pUDKH" @default.
- W2391640277 hasPublicationYear "2006" @default.
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