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- W2391663823 abstract "538 transformants from T-DNA mutant library of Monascus purpureus mediated by Agrobacterrium tumefaciens were used as materials,9 respresentative transformants with significant changes were selected through the colony and mirco-morphological observation,and verified by PCR amplification.The results showed that there were T-DNA insertions in transformants genome.Through stability examination,all 9 respresentative transformants could be steadily inherited.Based on this,two main metabolites of 9 respresentative transformants,which were pigments and γ-aminobutyric acid(GABA),were analyzed respectively by using the methods of ethanol extraction and paper chromatography.The results showed that the capacity of producing metabolites by transformants changed in different degrees compared with the original strain.It provided the foundation for the regulation mechanism of Monascus metabolite research." @default.
- W2391663823 created "2016-06-24" @default.
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- W2391663823 date "2012-01-01" @default.
- W2391663823 modified "2023-09-26" @default.
- W2391663823 title "Characteristic analysis and molecular verification of Monascus transformants" @default.
- W2391663823 hasPublicationYear "2012" @default.
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