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- W2391665306 abstract "Aim:To investigate the genotyping characteristic and drug resistance of the variable number tandem repeats(VNTR) of Mycobacterium tuberculosis clinical isolates in Weishi,Henan province with multi-locus VNTR analysis preliminarily.Methods:A total of 257 Mycobacterium tuberculosis clinical isolates selected from Weishi county randomly were cultured with Lowenstein-Jensen medium from June 2009 to June 2011.The drug susceptibility of Mycobacterium tuberculosis was determined by proportion method. The genotypes were identified by RD105 detection test and the polymorphism of 7 VNTR locus.The clustering of genotype was analyzed with BioNumerics.Results:Beijing family genotype accounted for 90.7%(233/257) by RD105 detection test.The VNTR genotypes showed obvious polymorphisms.The clustering of genotype showed that these strains could be categorized into 4 gene clusters(Ⅰ,Ⅱ,Ⅲ and Ⅳ)and 230 genotypes.Among them, 59.1% were cluster Ⅲ including 152 genotypes,which was the dominant genotype. The drug resistant result showed that there were 23 MDR-TB strains,accounting for 8.9%(23/257),any there were 63 drug resistant strains,accounting for 24.5%(63/257). The drug resistant strains distribution between Beijing family genotype and non-Beijing family genotype,as well as major population and non- major population had significant differences(χ2=2.412 and 1.954,P0.05).Conclusion:There are at least 4 clusters of VNTR genotype and cluster Ⅲ is the predominant prevalent strain in Weishi county. The drug resistance is still not optimistic." @default.
- W2391665306 created "2016-06-24" @default.
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- W2391665306 date "2012-01-01" @default.
- W2391665306 modified "2023-09-26" @default.
- W2391665306 title "Genotyping characteristic and drug resistance of Mycobacterium tuberculosis isolated in Weishi county of Henan province" @default.
- W2391665306 hasPublicationYear "2012" @default.
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