Matches in SemOpenAlex for { <https://semopenalex.org/work/W2391685324> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2391685324 endingPage "440" @default.
- W2391685324 startingPage "436" @default.
- W2391685324 abstract "Objective To construct Rab7 eukaryotic expression plasmid and analyze its expression in murine macrophage cell line RAW264.Methods The expression of Rab7 in tissues and cells were detected by RT-PCR.Using the cDNA of RAW264.7 cells as template,the open reading frame of Rab7 was amplified by primers designed according to the full-length sequence of Rab7 published in Genbank.The target gene was connected with the eukaryotic expression vector pcDNA3.1 to get pcDNA-Rab7.The recombinant eukaryotic vector of Rab7-pcDNA were transiently transfected into Raw264.7 cells by liposome,and the over expression state of Rab7 were indentified by RT-PCR and Western blot.Results Sequence analysis showed that the Rab7-pcDNA plasmid was 100% homologous with the sequence of Rab7 in Genbank.The expression of Rab7 was increased significantly as verified by RT-PCR and Western blot.Conclusion The eukaryotic expression vector of Rab7-pcDNA is successfully constructed.This study may lay a foundation for further elucidating the function of Rab7 in macrophages." @default.
- W2391685324 created "2016-06-24" @default.
- W2391685324 creator A5004993864 @default.
- W2391685324 creator A5009425178 @default.
- W2391685324 creator A5027164234 @default.
- W2391685324 creator A5039574044 @default.
- W2391685324 creator A5064727658 @default.
- W2391685324 creator A5090728786 @default.
- W2391685324 creator A5090814462 @default.
- W2391685324 date "2012-01-01" @default.
- W2391685324 modified "2023-09-22" @default.
- W2391685324 title "Cloning and expression of small G protein Rab7 in RAW264.7 cells" @default.
- W2391685324 hasPublicationYear "2012" @default.
- W2391685324 type Work @default.
- W2391685324 sameAs 2391685324 @default.
- W2391685324 citedByCount "1" @default.
- W2391685324 crossrefType "journal-article" @default.
- W2391685324 hasAuthorship W2391685324A5004993864 @default.
- W2391685324 hasAuthorship W2391685324A5009425178 @default.
- W2391685324 hasAuthorship W2391685324A5027164234 @default.
- W2391685324 hasAuthorship W2391685324A5039574044 @default.
- W2391685324 hasAuthorship W2391685324A5064727658 @default.
- W2391685324 hasAuthorship W2391685324A5090728786 @default.
- W2391685324 hasAuthorship W2391685324A5090814462 @default.
- W2391685324 hasConcept C104317684 @default.
- W2391685324 hasConcept C121050878 @default.
- W2391685324 hasConcept C153911025 @default.
- W2391685324 hasConcept C167625842 @default.
- W2391685324 hasConcept C187882448 @default.
- W2391685324 hasConcept C199360897 @default.
- W2391685324 hasConcept C22744801 @default.
- W2391685324 hasConcept C2776415932 @default.
- W2391685324 hasConcept C33161422 @default.
- W2391685324 hasConcept C40767141 @default.
- W2391685324 hasConcept C41008148 @default.
- W2391685324 hasConcept C47289529 @default.
- W2391685324 hasConcept C54009773 @default.
- W2391685324 hasConcept C54355233 @default.
- W2391685324 hasConcept C79029880 @default.
- W2391685324 hasConcept C86803240 @default.
- W2391685324 hasConcept C92087593 @default.
- W2391685324 hasConceptScore W2391685324C104317684 @default.
- W2391685324 hasConceptScore W2391685324C121050878 @default.
- W2391685324 hasConceptScore W2391685324C153911025 @default.
- W2391685324 hasConceptScore W2391685324C167625842 @default.
- W2391685324 hasConceptScore W2391685324C187882448 @default.
- W2391685324 hasConceptScore W2391685324C199360897 @default.
- W2391685324 hasConceptScore W2391685324C22744801 @default.
- W2391685324 hasConceptScore W2391685324C2776415932 @default.
- W2391685324 hasConceptScore W2391685324C33161422 @default.
- W2391685324 hasConceptScore W2391685324C40767141 @default.
- W2391685324 hasConceptScore W2391685324C41008148 @default.
- W2391685324 hasConceptScore W2391685324C47289529 @default.
- W2391685324 hasConceptScore W2391685324C54009773 @default.
- W2391685324 hasConceptScore W2391685324C54355233 @default.
- W2391685324 hasConceptScore W2391685324C79029880 @default.
- W2391685324 hasConceptScore W2391685324C86803240 @default.
- W2391685324 hasConceptScore W2391685324C92087593 @default.
- W2391685324 hasIssue "5" @default.
- W2391685324 hasLocation W23916853241 @default.
- W2391685324 hasOpenAccess W2391685324 @default.
- W2391685324 hasPrimaryLocation W23916853241 @default.
- W2391685324 hasRelatedWork W1229830084 @default.
- W2391685324 hasRelatedWork W2350547953 @default.
- W2391685324 hasRelatedWork W2351574077 @default.
- W2391685324 hasRelatedWork W2357609942 @default.
- W2391685324 hasRelatedWork W2359947648 @default.
- W2391685324 hasRelatedWork W2360086171 @default.
- W2391685324 hasRelatedWork W2361843014 @default.
- W2391685324 hasRelatedWork W2366818592 @default.
- W2391685324 hasRelatedWork W2367391397 @default.
- W2391685324 hasRelatedWork W2375033972 @default.
- W2391685324 hasRelatedWork W2375446097 @default.
- W2391685324 hasRelatedWork W2376215340 @default.
- W2391685324 hasRelatedWork W2376851738 @default.
- W2391685324 hasRelatedWork W2378833595 @default.
- W2391685324 hasRelatedWork W2378997015 @default.
- W2391685324 hasRelatedWork W2380303722 @default.
- W2391685324 hasRelatedWork W2380777667 @default.
- W2391685324 hasRelatedWork W2391255008 @default.
- W2391685324 hasRelatedWork W2393813340 @default.
- W2391685324 hasRelatedWork W2394189279 @default.
- W2391685324 hasVolume "46" @default.
- W2391685324 isParatext "false" @default.
- W2391685324 isRetracted "false" @default.
- W2391685324 magId "2391685324" @default.
- W2391685324 workType "article" @default.