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- W2391716900 abstract "The clavulanic acid-producing strain Streptomyces clavuligerus CA0726 with the yield of 233 μg/mL as parent strain was treated by UV irradiation and NTG approaches, the glycerol-tolerant mutants were screened and a high-titre strain CA0726-19 was obtained, with the productivity was 1645 μg/mL. It was stable in priductivity by continuous subcultures. The result from the fermentative experiments on the strain CA0726-19, showed that initial pH 7.0, inoculation volume 10% and fermentation temperature 28 ℃, the maximum potency of 2 397 μg/mL was obtained after fermentated for 96h in flask, an increase of 10.3 times compared with original strain. Preliminary experiment was carried out in 15 L fermentor. With inoculation volume 10%, ventilation 1∶1, rotation speed 500 r/min, fermentation temperature 28 ℃, the yield reached to 2 152 μg/mL after fermentated for 72 h ." @default.
- W2391716900 created "2016-06-24" @default.
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- W2391716900 date "2005-01-01" @default.
- W2391716900 modified "2023-09-28" @default.
- W2391716900 title "Breeding of clavulanic acid producing strain and optimization of fermentative conditions" @default.
- W2391716900 hasPublicationYear "2005" @default.
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